Protein Mass Spectrometry Sample Prep
Enzo Life Sciences FLUOR DE LYS-Succinyl Green (50µl)
The FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer (BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm). . Formulation: Solution in DMSO. Purity: ≥95% (HPLC). Long Term Storage: -80°C.
Electron Microscopy Sciences Trypsin Kit Trypsin Kit Small
Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. Reagent B (Buffer) is 15ml and Reagent E (Enzyme) is 2ml.
Waters Corp Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample
This basic kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, and the GlycoWorks Clean-up Module (excludes PN 186007988). It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 mL/vial Rapid PNGaseF Enzyme (1) 0.25 mL/vial Glycoworks Rapid Buffer (1) 3 vials of 3 mg/vial RapiGest SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 0.5 mL/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7) (4) 3 mL/vial Sample Diluent (DMF/ACN Mixture) (1) GlycoWorks HILIC µElution Plate
Waters Corp ProteinWorks Auto-eXpress Low 3 Digest Kit
The ProteinWorks Auto-eXpress Low 3 Digest Kit simplifies and accelerates the digestion of immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach. The kit includes a robust digest protocol and pre-measured, lot-traceable reagents in an automation friendly format for the denaturation, digestion and quench of proteins in biological matrices. This product is suitable for high protein content samples (0.2 - 1.0 mg total protein) and contains the following: ProtienWorks Low Digest Ambient Kit and ProteinWorks Low Digest Trypsin Kit
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit-Auto
GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit-Auto
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Starter Kit - Auto
GlycoWorks RapiFluor-MS N-Glycan Starter Kit - Auto
Waters Corp ProteinWorks eXpress Direct Digest Kit - Ambient Refill Kit
ProteinWorks eXpress Direct Digest Kit - Ambient Refill Kit
Waters Corp ProteinWorks eXpress Digest Kit - Ambient Refill Kit
ProteinWorks eXpress Digest Kit - Ambient Refill Kit
Bruker Daltonics MTP SMALLANCHOR 384 BC
MTP SmallAnchor 384 BC MTP SmallAnchor 384 BC: MALDI target for scout MTP ion sources with 384 SmallAnchors (approx. 400 μm diameter) for sample preparation plus 96 CalibAnchors (additional anchor for external calibration). - frame (#8074115) required - dedicated for autoflex and ultraflex series mass spectrometers - compatible with Dual ESI/MALDI Source
